Background Osteoarthritis (OA) is a common disease of the joints among old populace until today. The treatment possibilities and roles of miRNA and long non-coding RNA (lncRNA) in therapy of OA has previously been explored. However, the functional roles of Long noncoding RNA KCNQ1OT1 and miRNA let-7a-5p on Osteoarthritis development and progression remains unclear. This study aimed at investigating the influence of KCNQ1OT1 on let-7a-5p in moderation of OA development and advancement.\nMaterials and Methods RT-qPCR examined expression of KCNQ1OT1and let-7a-5p in cultured human primary chondrocyte cell lines. Cell transfection overexpressed or knocked down the genes and CCK-8 assay measured cell viability in the proliferation biomarkers Ki87 and PCNA. While caspase-8 and caspase-3 activity determined rate of apoptosis. Furthermore, luciferase assay analyzed the luciferase activity and western blotting analysis determined the protein expression of KCNQ1OT1 and let-7a-5p in proliferation and apoptosis biomarkers.\nResults The results demonstrated that KCNQ1OT1 is upregulated in OA-mimic cells and promotes the cell viability. KCNQ1OT1 knockdown suppresses cell viability of OA cells. Furthermore KCNQ1OT1 directly binds the 3\'-UTR of let-7a-5p to negatively regulate let-7a-5p expression and OA progression. While upregulated let-7a-5p abolishes the proliferation effect of KCNQ1OT1 in OA cells.\nConclusion In summary, our study provides further insights into the underlying molecular mechanisms of KCNQ1OT1 and let-7a-5p suggesting a novel therapeutic approach to OA
In this article, we are going to study the linear support vectors and their performance in the related classification issues. Using the linear support vectors (SVM\'s) in the classification issues is a new approach that in recent years is considered by many scientists. It was used in a wide range of applications including OCR, Handwriting recognition, guidance signs diagnosis and etc. SVM approach is in a way that in the training phase, it is tried to choose the limit of decision-making (Decision Boundary) is such a way that its minimum distance to each of the considered categories stays maximum. This kind of choice helps our decision in practice to tolerate the noisy condition very well and has a good response. This way of selecting the boundary is based on the points that are named as support vectors. At first we study the concepts such as generalization of a pattern recognition machine and then the VC dimension that has a great application in the concept of classification machines. And then we describe the linear and non-linear support vectors and Kernel functions. And eventually, we will study the VC dimension for some of these functions.
Female foeticide is a deliberate and intentional act of aborting a foetus because it is a female. Female foetuses are selectively aborted after detecting sex of the foetus. As a result 10 lakh girls are missing from the Indian population. The problem of the “missing” girl child or the practice of female foeticide is not uncommon to the country. The present study focused on the causes of female foeticide in Kashmir valley. The research was carried out by survey of 400 families in 10 districts of Kashmir valley viz Srinagar, Anantnag, Baramulla, Sopore, Shopian, Bandipora, Kulgam, Budgam, Ganderbal and Pulwama. The data was collected with the help of structured questionnaire along with interviews with the respective families. The results of the study revealed that major cause of female foeticide is large amount of money required for daughter’s marriage than son’s marriage in the form of dowry. If a girl is educated, the problem becomes even worse because higher the education level, higher the expenditure on marriage to maintain the status. Results also show that female foeticide is due to last rites of parents can be performed by a son only. Besides religious consideration, economic, social and emotional desires favour males. Parents expect sons but not daughters.
The purpose of this study was to verify the effectiveness of the proposed program of computer games on reducing Attention-deficit disorder in certain categories of people with special needs. The sample of the study consisted of (10) children with learning disabilities, (10) deaf children and (10) children with mild mental disabilities able to learn in primary school from the first grade to sixth grade in Jeddah city in Saudi Arabia. The students were selected by applying Diagnostic and Statistical Manual of Mental Disorders scale, fourth edition, text revision (DSM-IV-TR®) for attention deficit and hyperactivity. The researcher used electronic attention disorder scale (Alert test). The results indicated significant differences between the ranks\' scores of the experimental group of students with special needs in the certain categories before, and after the application of the program in favor of posttest measurement.
Objectives: The objective of this study was to determine, the relationship of 25-hydroxyvitamin D (25(OH)D) serum level with the coronary artery disease (CAD) severity and prognosis in patients with acute coronary syndrome (ACS) during a three-year follow up period. \nMethods: The study included 60 ACS patients hospitalized at cardiology department for ACS between March 2012 and October 2012 and 60 age- and sex-matched control patients without ACS. Standard laboratory testing and vitamin D determination were performed in all study patients. In addition, ACS patients underwent coronarography and were followed-up for 36 months of ACS for major adverse cardiac events (MACE). \nResults: Patients with ACS had a statistically significantly lower mean 25(OH)D level as compared with control group (35.19 nmol/L vs. 58.08 nmol/L, p<0.001). The lowest mean level of 25(OH)D was recorded in diabetic patients with ACS (30.45 nmol/L). ACS patients were divided into three subgroups according to coronarography findings: single vessel, double vessel and triple vessel CAD with respective serum levels of 25(OH)D of 36.44 nmol/L, 33.65 nmol/L and 31.70 nmol/L. During 36-month follow up, the event-free survival rate was 60% in the ACS group. The ACS patients having sustained MACE during follow up had low serum level of 25(OH)D in the acute phase; however, the difference from ACS patients without MACE during follow up did not reach statistical significance (32.64 nmol/L vs. 37.01 nmol/L).\nConclusion: This study definitely showed significantly lower concentration of vitamin D among ACS patients, with no effect on 3-year mortality within that group related to baseline vitamin D concentration. Whether vitamin D is an important part of still unsolved cardiovascular puzzle, or just a prognostic factor for cardiovascular disease is left for large prospective trials to answer. Additionaly, the connection between diabetes, vitamin D metabolism and coronary artery disease should be investigated more thoroughly in the future, since diabetics showed lowest concentrations among all.