In this study, the distribution of study variables including age, gender, residence, family history, smoking, treatment with any topical antibiotic and treatment with any systemic drug among patients with Acne vulgaris were studied, it was showed that, the age range between 18 to 22 years was considers more infection with Acne vulgaris in rate 65% than other ages range, and 48% of the infection was female in these ages range, while the age range between 23-25 was male infected with Acne vulgaris in rate (36%). In addition, 55% of infection with Acne vulgaris were lived in urban compared with 45% of infection in rural. According to family history, the results showed that, 63% of these infection were people with an existing family history, and 57% were no smoking. However, 93% of these patients were treated with topical treatment and 84% were treated with systemic drug. However, severity of Acne vulgaris infected disease was classified according to symptoms, the results showed that, the mild infection were appeared in 22%, moderate infection were appeared in 63% and 15 % were appeared as severe infection. A total 100 skin swab samples of patients suffering from Acne vulgaris, the results showed that, out of 100 samples, 83(83%) give positive culture, while 17(17%) samples were negative culture. Out of 83 positive culture on different types of growth media, and the bacterial was identified according to gram stain, the results showed that, 65(78.31%) was classified as gram positive and gram negative bacteria, while 18(27.69%) were related to other types of causative agents. Staphylococcus aurous was considered the most commonly bacterial isolates from patients with Acne vulgaris in rate (38%), followed by Staphylococcus epidermidis in rate (27.8%), (18.6%) were related to Propionibacterium acnes, micrococcus spp. in rate (10.9%), and Corynebacterium in rate (4.7%). To confirm the isolates of bacteria was used GP-GN ID cards with 64 biochemical tests were used in the computerized Compact Vitek-2 system. The results demonstrated that all (65) isolates were confirmed with ID massage confidence level ranging excellent (probability percentage from 94 to 99.7%, this technique was characterized by fast detection of bacteria. it was found that, all bacterial isolates were identified by culture media and biochemical test, were identified in rate (100%) by this system.