This study examined the Hall current effects and thermal radiation on magnetohydrodynamic (MHD) flow over a vertical accelerated plate. The effects of Hall current and thermal radiation are studied in a vertical plate. The nondimensional partial differential equations of the model are solved analytically using the Laplace transform method with the help of the error function (Erf); thus, the expressions for the velocity field, temperature field, concentration field, skin friction coefficient, Nusselt number, and Sherwood number are obtained. The impact of physical parameters such as R (radiation parameter), m (Hall parameter), and λ (exponential parameter) on these studies are discussed graphically in detail.

Objective: To investigate the effect of heme oxygenase-1 (HO-1) on the biological behavior and oxidative stress damage of osteoblasts. Methods: The skull osteoblasts were divided into three groups: negative control group (no treatment), positive control group (treated with H2O2) and experimental group (with H2O2 and transfection of HO-1). The expressions of biological behavior indexes, oxidative stress indexes and related protein indexes were detected. Results: The biological behavior, oxidative stress injury and protein indexes of osteoblasts were improved after HO-1 intervention. Conclusion: HO-1 can inhibit the oxidative stress damage of osteoblasts induced by H2O2, as well as promote the proliferation and inhibit the apoptosis of osteoblasts.

Background: The demand for platelets in clinical patients is increasing year by year. However, the urgent infusion demand is often not met. \nObjectives: To compare the coagulation function of frozen and fresh platelets in vitro and to investigate the effect of shock storage or cryopreservation on platelet function.\nMethods: Platelets of healthy people were isolated, divided into 2 equal parts, respectively, and stored as shock storage and cryopreservation. Using 5% DMSO as protective agent, the platelets were frozen at -80℃, then stored for 4 days and cryopreserved for 4 days. The maximum amplitude (MA) was determined using thromboelastogram, and the CD62P count was detected using flow cytometry. \nResults: The MA of frozen platelet (30.80±6.27 mm) was greater than that of fresh platelets (28.94±5.82 mm), indicating that the coagulation function of frozen platelets in vitro was stronger than that of fresh platelets, while the CD62P content of frozen platelets (29.50±3.06%) was smaller than that of fresh platelets (55.25±4.09%), indicating that the aggregation function of frozen platelets was stronger than that of fresh platelets. \nConclusions: Frozen-stored platelets have stronger coagulation function than fresh platelets in vitro. Platelets can be stored for a long time after deep-frozen to meet the increasing clinical demand for platelets.

Rice (Oryza sativa L.) is one of the world\'s most important crops, and the primary source of calories for more than three billion people around the world, especially in Asia. Currently, atmospheric carbon dioxide (CO2) concentration is 416 µmol mol-1 and increasing rapidly due to industrialization which is the main cause of global warming. Current climate change and increasing CO2 concentration in the atmosphere have varying global impacts on crop performance. Evidently, an increase in CO2 has been shown to improve rice productivity. As CO2 is one of the limiting factors in photosynthesis, any addition of this gas can increase carboxylation activity, hence increases productivity and yield. Elevated CO2 (eCO2) had significant impacts on C3 crop\'s productivity by increasing photosynthesis, biomass, and grain yield. Plants cultivated under eCO2 conditions demonstrated better development, and photosynthesis, lower transpiration, improve water efficiency, decreased inorganic nutrition concentration, increased plant hormone, and compact stomatal density than the plants grown under ambient CO2 (aCO2) conditions. This review discusses the effects of eCO2 on rice plant photosynthesis and growth. The review also describes that eCO2 increased yield components of rice plants. And finally, the current review emphasizes the grain quality of rice that was shown to be negatively affected by eCO2. The synthesis of all this information is helpful to the researchers, advisors of rice farmers, and policymakers to provide a favorable plan by using eCO2 to increase rice plant growth and yield through enrich free-air CO2 enrichment (FACE) system for the rice plant to produce enough food for the increasing population of the world.

Background: Primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL) lacks specific clinical manifestations and its malignancy renders prognostication and choice of treatment strategy difficult. The aim of this study was to evaluate microRNA (miR)-21 as potential non-invasive biomarkers for prognosis in PGI-DLBCL patients. \nMethods: Serum miR-21 expression in de novo PGI-DLBCL patients, consecutively enrolled for this study, was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Relative expression was calculated using the comparative Ct method. Statistical significance was determined using the Mann-Whitney rank sum and Fisher’s exact test. Survival analysis was conducted using Kaplan-Meier method.\nResults: Compared with healthy controls, serum miR-21 levels were significantly elevated in the PGI-DLBCL patients (n=156). High expression level of serum miR-21 at diagnosis was associated with a worse progression-free survival (PFS) (30 (9-42) vs 42 (12-52) months in high and low miR-21 groups) and overall survival (OS) (35 (15-52) vs 48 (17-61) months in high and low miR-21 groups) and was independent risk factor for PFS and OS (hazard ratios 4.345 and 3.311, respectively). Furthermore, Bcl-2, Bcl-6 and Ki-67 were independently and positively associated with miR-21 expression.\nConclusions: Our results suggest that miR-21 is a potential prognostic marker to predict clinical outcome in PGI-DLBCL patients and high miR-21 level is associated with poor outcome.

This study was conducted to examine the association between the methylation of CDKN2A (p16) gene and relevant receptor expression in breast cancer (BC) for better diagnosis of the disease. We used PCR specifically designed for methylation (MSP) to detect the methylation in the gene in 140 tissue samples and 128 serum samples from BC patients treated at our center. The streptavidin- SP method of immunoperoxidase histochemistry was used to detect ER, PR and HER2. The relationships between these parameters and BC were analyzed. In the tissue and serum samples, the overall methylation rates of the p16 gene were 29.3% (41/140) and 28.1% (36/128). In ER, PR and HER2-negative patients, the methylation rate of the p16 gene was significantly greater than that in non-triple negative patients (P < 0.01, 93.3% (30/34) vs 21.0% (22 /105). The methylation of the gene was negatively correlated with ER, PR and HER2 [removed]r = -0.564, -0.664 and – 0.724, respectively, P < 0.05), but not correlated with cancer grade and metastasis. Receiver operator characteristic (ROC) curve analysis that p16 gene methylation had a significant predictive value for TNBC with an AUC of 0.825. Therefore, p16 gene methylation is a common molecular change associated TNBC subtype and has clinical value for early diagnosis, treatment and prognosis of BC.

This study was conducted to investigate the changes of Clara cell protein 16 (CC16) and surfactant protein D (SP-D) levels in serum and bronchoalveolar lavage fluid (BALF) in silicotic rats and to explore their potential as early biomarker for silicosis. Pulmonary fibrosis models of rats were constructed by exposing them to silica particles. BALF and serum were collected to determine CC16 and SP-D levels using enzyme-linked immunosorbent assay (ELISA) at different times after the exposure. Hydroxyproline (HYP) level in BALF and CC16 level in the lung tissues were also measured immunohistochemistrially. The BALF levels of CC16 decreased from 49.65 to 38.02 ng/mg after the rats were exposed to silica for 3 and 28 days, which were all significantly lower as compared with the controls (P < 0.05), where the levels remained barely changed during the same period (61.27 to 56.76 ng / mg)．The serum CC16 also showed the similar decrease from 9.8 ng/ml to 8.78 ng / ml during the period, while in the controls, the serum CC16 levels remained constantly between 11.04 and 10.96 ng / ml．The levels of SP-D in the serum of silica-exposed rats did not decreased as compared with the controls and BALF SP-D presented a parabolic curve change with silica exposure. Immunohistochemical examinations showed that the lung Clara cells were severely damaged and CC16 expression was obviously decreased after silica exposure. BALF HYP level was higher in silica-exposed rats than control only when the exposure was at 50 mg/ml. Our work demonstrates that expressions of CC16 and SP-D are pulmonary tissue-specific and CC16 expression is down-regulated as a result of silica-exposure. The significant relationship between CC16 and silica dose indicates that CC16 may be exploited as early biomarker to assess silica-induced pulmonary fibrosis．

Objective: To investigate the diagnostic value of serum ferritin (SF) with four tumor markers for early lung cancer in the elderly. \nMaterials and Methods: A total of 370 senile patients with early stage lung cancer, 120 senile patients with benign lung disease and 100 healthy elderlys were analysed for serum SF, carbohydrate antigen (CA)125, carcino-embryonic antigen (CEA), neuron-specific enolase (NSE) and cytokeratin fragment (CYFRA) 21-1. The specificity and sensitivity of these paramters in diagosis of the lung cancer were compared and their diagonsitic values were analyzed using the receiver operating characteristic (ROC) curve.\nResults: Serum levels of SF, CA125, CEA, NSE and CYFRA21-1 were significantly higher in patients with lung caner than those with benign lung disease and healthy participants, while there was no significant difference in these levels between patients with benign lung disease and healthy controls. Serum levels of SF and NSE were the highest in patients with small cell lung cancer, and CA125 and CEA levels were the highest in patients with adenocarcinoma and the highest level of CYFRA21-1 was found in patients with squamous cell carcinoma. Compared with diagnosis using single parameters, diagnosis with five parameters significantly incrased the specificity, but not the specificity (P > 0.05). The areas under ROC curve of SF, CA125, CEA, NSE and CYFRA21-1 were 0.82, 0.79, 0.78, 0.75 and 0.79 for SF, CA125, CEA, NSE and CYFRA21-1, respectively, and 0. 95 for the five parameters in the diagnosis of early lung cancer in the elderly.\nConclusions: Compared with diagnosis using single parameters, the combined use of SF with the four markers significantly increased the sensitivity and diagnositic value of the diagnosis method.